Mycobacterology laboratory research will include TB immuno-pathogenesis, primarily TB genomic study, and recombinant BCG or TB vaccine development.
TB research is still needed to provide a solid foundation of understanding of the pathogenesis and development of drug resistance in TB.
1.Surveillance of epidemiology and drug resistance of TB in Taiwan.
2.Understanding the epidemiology that contribute to the development and spread of
Drug resistant strains of Mtb
3.Study the host immunity and markers that signal transition from latent to active TB.
4.Understanding the basic biology and immunology of host and pathogen that underlie
the development and spread of drug-resistant strains of Mtb.
5.Identification of candidate virulence factor for the pathogenesis of MTB infection and
Research Activities & Accomplishment
In general, the public health impact of vaccination programs with new TB vaccines will be dependent crucially on (i) the diversity of circulating Mtb strains, (ii) the mechanisms of competition between strains, and (iii) the strain specificity of these vaccines. In this study, we have revealed the distribution of MTB in Taiwan is closely associated with the ethnicity and migratory population at different historical periods. These results have implications for tuberculosis control efforts, especially for the development of new vaccines.
1.To study the molecular epidemiology of MTB in Taiwan.
2.To fully explore gene variability within the clinical isolate. We will perform the complete genome sequencing and annotation of a clinical isolate of M. tuberculosis strain from different ethnic population, in an attempt to correlate genotypic changes with strain phenotype.
3.To modify BCG by recombinant DNA technology to produce a new live vaccine that expresses immunodominant antigen and/or cytokine with efficacy superior to that of BCG.
Publications: (* Dou HY as corresponding author)
1.Chen YY, Chang JR, Huang WF, Kuo SC, Yeh JJ, Lee JJ, Jang CS, Sun JR, Chiueh TS, Su IJ, Dou HY*. Molecular epidemiology of Mycobacterium tuberculosis in aboriginal peoples of Taiwan, 2006-2011. J Infect. 2013 Dec 24. pii: S0163-4453(13)00380-0
2.Huang L, Chen TL, Lee YT, Lee MH, Kuo SC, Yu KW, Dou HY, Fung CP. Risk factors for imipenem-nonsusceptible Acinetobacter nosocomialis bloodstream infection. J Microbiol Immunol Infect. 2013 Apr 30. pii: S1684-1182(13)00028-5.
3.Lee MH, Chen TL, Lee YT, Huang L, Kuo SC, Yu KW, Hsueh PR, Dou HY, Su IJ, Fung CP. Dissemination of multidrug-resistant Acinetobacter baumannii carrying BlaOxA-23 from hospitals in central Taiwan. J Microbiol Immunol Infect. 2013 Dec;46(6):419-24.
4.Chang JR, Chen YY, Huang TS, Huang WF, Kuo SC, Tseng FC, Su IJ, Lin CH, Chen YS, Sun JR, Chiueh TS, Dou HY*. Clonal expansion of both modern and ancient genotypes of Mycobacterium tuberculosis in southern Taiwan. PLoS One. 2012;7(8):e43018.
5.Chen YY, Chang JR, Huang WF, Kuo SC, Su IJ, Sun JR, Chiueh TS, Huang TS, Chen YS, Dou HY*. Genetic diversity of the Mycobacterium tuberculosis Beijing family based on SNP and VNTR typing profiles in Asian countries. PLoS One. 2012;7(7):e39792.
6.Weng CY, Ho CM, Dou HY, Ho MW, Lin HS, Chang HL, Li JY, Lin TH, Tien N, Lu JJ. Molecular typing of Mycobacterium tuberculosis isolated from adult patients with tubercular spondylitis. J Microbiol Immunol Infect. 2013 Feb;46(1):19-23.
7.de Beer JL, Kremer K, Ködmön C, Supply P, van Soolingen D; Global Network for the Molecular Surveillance of Tuberculosis 2009. First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains. J Clin Microbiol. 2012 Mar;50(3):662-9.
8.Lin CW, Su IJ, Chang JR, Chen YY, Lu JJ, Dou HY*. Recombinant BCG coexpressing Ag85B, CFP10, and interleukin-12 induces multifunctional Th1 and memory T cells in mice. APMIS. 2012 Jan;120(1):72-82.
9.Huang SF, Su WJ, Dou HY, Feng JY, Lee YC, Huang RM, Lin CH, Hwang JJ, Lee JJ, Yu MC. Association of Mycobacterium tuberculosis genotypes and clinical and epidemiological features - a multi-center study in Taiwan. Infect Genet Evol. 2012 Jan;12(1):28-37.
10.Sun JR, Dou HY, Lee SY, Chiueh TS, Lu JJ. Epidemiological studies of Beijing strains of Mycobacterium tuberculosis from Taipei and other Asian cities based on MIRU profiles. APMIS. 2011 Sep;119(9):581-7.
11.Chang JT, Dou HY, Yen CL, Wu YH, Huang RM, Lin HJ, Su IJ, Shieh CC. Effect of type 2 diabetes mellitus on the clinical severity and treatment outcome in patients with pulmonary tuberculosis: a potential role in the emergence of multidrug-resistance. J Formos Med Assoc. 2011 Jun;110(6):372-81.
12.Dou HY, Huang SC, Su IJ*. Prevalence of Mycobacterium tuberculosis in Taiwan: A Model for Strain Evolution Linked to Population Migration. Int J Evol Biol. 2011 Jan 17;2011:937434.
13.Chang JR, Lin CH, Tsai SF, Su IJ, Tseng FC, Chen YT, Chiueh TS, Sun JR, Huang TS, Chen YS, Dou HY*. Genotypic analysis of genes associated with transmission and drug resistance in the Beijing lineage of Mycobacterium tuberculosis. Clin Microbiol Infect. 2011 Sep;17(9):1391-6.
14.Lee SS, Chou KJ, Dou HY, Huang TS, Ni YY, Fang HC, Tsai HC, Sy CL, Chen JK, Wu KS, Wang YH, Lin HH, Chen YS. High prevalence of latent tuberculosis infection in dialysis patients using the interferon-gamma release assay and tuberculin skin test. Clin J Am Soc Nephrol. 2010 Aug;5(8):1451-7.
15.Dou HY, Lu JJ, Lin CW, Chang JR, Sun JR, Su IJ*. Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis solates. J Microbiol Methods. 2009 Apr;77(1):127-9.
16.Dou HY, Tseng FC, Lin CW, Chang JR, Sun JR, Tsai WS, Lee SY, Su IJ, Lu JJ. Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei. BMC Infect Dis. 2008 Dec 22;8:170. doi: 10.1186/1471-2334-8-170.
17.Sun JR, Lee SY, Dou HY, Lu JJ. Using a multiplex polymerase chain reaction for the identification of Beijing strains of Mycobacterium tuberculosis. Eur J Clin Microbiol Infect Dis. 2009 Jan;28(1):105-7.Dou HY, Tseng FC, Lu JJ, Jou R, Tsai SF, Chang JR, Lin CW, Miu WC, Su IJ*. Associations of Mycobacterium tuberculosis genotypes with different ethnic and migratory populations in Taiwan. Infect Genet Evol. 2008 May;8(3):323-30.